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control igg  (Proteintech)


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    Structured Review

    Proteintech control igg
    Control Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igg+control/IgG+control+Antibody/pmc13019968-295-3-5
    Average 96 stars, based on 1275 article reviews
    control igg - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Androgen receptor-dependent DRAM1 activation drives autophagic resistance to BRAF inhibitors in BRAFV600-mutant melanoma
    Article Snippet: Chromatin was fragmented to ~500 bp using a Covaris S220 sonicator (20% duty cycle, 200 cycles/burst, 15 cycles). .. Lysates were immunoprecipitated overnight at 4 °C with 5 μg anti-AR (CST, #5153) or IgG control (Proteintech, #30000-0-AP), followed by 3 h incubation with Protein G Magnetic Beads (CST, #9006). .. After crosslink reversal and DNA purification (Tiangen, #DP214), AR occupancy at the DRAM1 promoter was quantified via qPCR (SYBR Green) with primers targeting predicted response elements (ΔCt normalized to Input DNA).

    Article Title: Androgen receptor-dependent DRAM1 activation drives autophagic resistance to BRAF inhibitors in BRAFV600-mutant melanoma.
    Article Snippet: Chromatin was fragmented to ~500 bp using a Covaris S220 sonicator (20% duty cycle, 200 cycles/burst, 15 cycles). .. Lysates were immunoprecipitated overnight at 4 °C with 5 μg anti-AR (CST, #5153) or IgG control (Proteintech, #30000-0- AP), followed by 3 h incubation with Protein G Magnetic Beads (CST, #9006). .. After crosslink reversal and DNA purification (Tiangen, #DP214), AR occupancy at the DRAM1 promoter was quantified via qPCR (SYBR Green) with primers targeting predicted response elements (ΔCt normalized to Input DNA).

    Article Title: Metformin attenuates HSV-1-induced neuropathic pain by restoring Sirt3-mediated mitophagy.
    Article Snippet: .. Immunoprecipitation was performed overnight at 4°C using anti-CREB antibody (Proteintech #12208-1-AP) or IgG control, followed by Protein A/G magnetic bead capture. ..

    Article Title: Functional landscape of non-canonical open reading frames in coordinating cell fate.
    Article Snippet: Article Functional landscape of non-canonical open reading frames in coordinating cell fate

    Control:

    Article Title: Androgen receptor-dependent DRAM1 activation drives autophagic resistance to BRAF inhibitors in BRAFV600-mutant melanoma
    Article Snippet: Chromatin was fragmented to ~500 bp using a Covaris S220 sonicator (20% duty cycle, 200 cycles/burst, 15 cycles). .. Lysates were immunoprecipitated overnight at 4 °C with 5 μg anti-AR (CST, #5153) or IgG control (Proteintech, #30000-0-AP), followed by 3 h incubation with Protein G Magnetic Beads (CST, #9006). .. After crosslink reversal and DNA purification (Tiangen, #DP214), AR occupancy at the DRAM1 promoter was quantified via qPCR (SYBR Green) with primers targeting predicted response elements (ΔCt normalized to Input DNA).

    Article Title: L-cystine alleviates necrotizing enterocolitis by regulating ferroptosis and Th17 cell differentiation via the IL-6/STAT3 pathway.
    Article Snippet: Communications Biology | (2026) 9:162 11 RNA Immunoprecipitation (RIP)-qPCR The interaction between RC3H1 and IL-6 mRNA was analyzed using a commercial kit (RIP-12RXN, Sigma-Aldrich). .. Magnetic beads were preincubated with 5 μg of anti-RC3H1 antibody (ab70195, Abcam) or speciesmatched IgG control (Proteintech) and mixed with cell lysates overnight at 4 °C. ..

    Article Title: Androgen receptor-dependent DRAM1 activation drives autophagic resistance to BRAF inhibitors in BRAFV600-mutant melanoma.
    Article Snippet: Chromatin was fragmented to ~500 bp using a Covaris S220 sonicator (20% duty cycle, 200 cycles/burst, 15 cycles). .. Lysates were immunoprecipitated overnight at 4 °C with 5 μg anti-AR (CST, #5153) or IgG control (Proteintech, #30000-0- AP), followed by 3 h incubation with Protein G Magnetic Beads (CST, #9006). .. After crosslink reversal and DNA purification (Tiangen, #DP214), AR occupancy at the DRAM1 promoter was quantified via qPCR (SYBR Green) with primers targeting predicted response elements (ΔCt normalized to Input DNA).

    Article Title: Metformin attenuates HSV-1-induced neuropathic pain by restoring Sirt3-mediated mitophagy.
    Article Snippet: .. Immunoprecipitation was performed overnight at 4°C using anti-CREB antibody (Proteintech #12208-1-AP) or IgG control, followed by Protein A/G magnetic bead capture. ..

    Article Title: Transcriptomic landscape of transposable elements reveals LTR7 - PLAAT4 as a potential oncogene and therapeutic target in pancreatic adenocarcinoma
    Article Snippet: ChIP–PCR was performed using the CUT&RUN approach with the Hyperactive pG-MNase CUT&RUN kit (Vazyme, #HD101-01) in AsPC-1 cells. .. Briefly, 1 × 10 5 cells were bound to ConA beads, incubated overnight with FOXM1 antibody (Proteintech, #13147-1-AP, RRID:AB_2106213) or IgG control (Proteintech, #30000-0-AP, RRID: AB_2819035), and digested with MNase. ..

    Article Title: Functional landscape of non-canonical open reading frames in coordinating cell fate.
    Article Snippet: Article Functional landscape of non-canonical open reading frames in coordinating cell fate

    Article Title: Silencing of lncRNA TFAP2A-AS1 attenuates the development of acute coronary syndrome by inhibiting TFAP2A expression
    Article Snippet: Invitrogen; Thermo Fisher Scientific, Inc. supplied the PrimeScript RT reagent and SYBR Green Premix for the experiment. .. Primary antibodies TFAP2A (cat. no. 13019-3-AP), IgG control (cat. no. 30000-0-AP) and GAPDH (cat. no. 60004-1-Ig) were purchased from Proteintech Group, Inc., along with horseradish peroxidase (HRP)-conjugated secondary antibodies (cat. no. SA00001-2). .. The Magna RIP RNA-Binding Protein Immunoprecipitation kit (cat. no. 17-700) was obtained from MilliporeSigma.

    Article Title: NR6A1 is essential for neural crest cell specification, formation and survival.
    Article Snippet: Antibodies used were: NR6A1 (Proteintech Ca. .. No. 12712-1-AP) and IgG control (Proteintech Ca. ..

    Incubation:

    Article Title: Androgen receptor-dependent DRAM1 activation drives autophagic resistance to BRAF inhibitors in BRAFV600-mutant melanoma
    Article Snippet: Chromatin was fragmented to ~500 bp using a Covaris S220 sonicator (20% duty cycle, 200 cycles/burst, 15 cycles). .. Lysates were immunoprecipitated overnight at 4 °C with 5 μg anti-AR (CST, #5153) or IgG control (Proteintech, #30000-0-AP), followed by 3 h incubation with Protein G Magnetic Beads (CST, #9006). .. After crosslink reversal and DNA purification (Tiangen, #DP214), AR occupancy at the DRAM1 promoter was quantified via qPCR (SYBR Green) with primers targeting predicted response elements (ΔCt normalized to Input DNA).

    Article Title: Androgen receptor-dependent DRAM1 activation drives autophagic resistance to BRAF inhibitors in BRAFV600-mutant melanoma.
    Article Snippet: Chromatin was fragmented to ~500 bp using a Covaris S220 sonicator (20% duty cycle, 200 cycles/burst, 15 cycles). .. Lysates were immunoprecipitated overnight at 4 °C with 5 μg anti-AR (CST, #5153) or IgG control (Proteintech, #30000-0- AP), followed by 3 h incubation with Protein G Magnetic Beads (CST, #9006). .. After crosslink reversal and DNA purification (Tiangen, #DP214), AR occupancy at the DRAM1 promoter was quantified via qPCR (SYBR Green) with primers targeting predicted response elements (ΔCt normalized to Input DNA).

    Article Title: Transcriptomic landscape of transposable elements reveals LTR7 - PLAAT4 as a potential oncogene and therapeutic target in pancreatic adenocarcinoma
    Article Snippet: ChIP–PCR was performed using the CUT&RUN approach with the Hyperactive pG-MNase CUT&RUN kit (Vazyme, #HD101-01) in AsPC-1 cells. .. Briefly, 1 × 10 5 cells were bound to ConA beads, incubated overnight with FOXM1 antibody (Proteintech, #13147-1-AP, RRID:AB_2106213) or IgG control (Proteintech, #30000-0-AP, RRID: AB_2819035), and digested with MNase. ..

    Magnetic Beads:

    Article Title: Androgen receptor-dependent DRAM1 activation drives autophagic resistance to BRAF inhibitors in BRAFV600-mutant melanoma
    Article Snippet: Chromatin was fragmented to ~500 bp using a Covaris S220 sonicator (20% duty cycle, 200 cycles/burst, 15 cycles). .. Lysates were immunoprecipitated overnight at 4 °C with 5 μg anti-AR (CST, #5153) or IgG control (Proteintech, #30000-0-AP), followed by 3 h incubation with Protein G Magnetic Beads (CST, #9006). .. After crosslink reversal and DNA purification (Tiangen, #DP214), AR occupancy at the DRAM1 promoter was quantified via qPCR (SYBR Green) with primers targeting predicted response elements (ΔCt normalized to Input DNA).

    Article Title: L-cystine alleviates necrotizing enterocolitis by regulating ferroptosis and Th17 cell differentiation via the IL-6/STAT3 pathway.
    Article Snippet: Communications Biology | (2026) 9:162 11 RNA Immunoprecipitation (RIP)-qPCR The interaction between RC3H1 and IL-6 mRNA was analyzed using a commercial kit (RIP-12RXN, Sigma-Aldrich). .. Magnetic beads were preincubated with 5 μg of anti-RC3H1 antibody (ab70195, Abcam) or speciesmatched IgG control (Proteintech) and mixed with cell lysates overnight at 4 °C. ..

    Article Title: Androgen receptor-dependent DRAM1 activation drives autophagic resistance to BRAF inhibitors in BRAFV600-mutant melanoma.
    Article Snippet: Chromatin was fragmented to ~500 bp using a Covaris S220 sonicator (20% duty cycle, 200 cycles/burst, 15 cycles). .. Lysates were immunoprecipitated overnight at 4 °C with 5 μg anti-AR (CST, #5153) or IgG control (Proteintech, #30000-0- AP), followed by 3 h incubation with Protein G Magnetic Beads (CST, #9006). .. After crosslink reversal and DNA purification (Tiangen, #DP214), AR occupancy at the DRAM1 promoter was quantified via qPCR (SYBR Green) with primers targeting predicted response elements (ΔCt normalized to Input DNA).



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